Journal: The Journal of International Medical Research

Article Title: Pachymic acid alleviates lipopolysaccharide-induced cystitis glandularis by inhibiting ferroptosis

doi: 10.1177/03000605251396755

Figure Lengend Snippet: Therapeutic effect of PA on LPS-induced CG. (a) Histopathological sections of bladder tissues from each group were observed via H&E staining. The magnified regions revealed the epithelial layer of the bladder. In the LPS group, distinct formation of Brunn’s nests was observed, accompanied by epithelial edema and increased inflammatory cell infiltration. Following treatment with Fer-1 and PA, Brunn’s nests disappeared, epithelial edema was alleviated, and inflammatory cell infiltration was reduced (Scale bar = 100 μm). (b) At 400× magnification, histopathological features across multiple fields of view were observed. The severity of inflammation was scored and visualized as a heatmap, where columns represent three individual animals per group, and rows correspond to five distinct microscopic fields from each animal. Additionally, statistical analysis of the inflammatory scores was performed. (c) to (e) Levels of IL-1β, IL-6, and TNF-α in bladder tissues from each group of rats. The above data are presented as mean value ± SD, ns means p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. CG: cystitis glandularis; H&E: hematoxylin and eosin; LPS: lipopolysaccharide; PA: pachymic acid.

Article Snippet: Human bladder epithelial cells (SV40-immortalized human urothelial cells-1 (SV-HUC-1)) and Sprague–Dawley rat bladders were treated with lipopolysaccharide, with pachymic acid and ferrostatin-1 used as interventions.

Techniques: Staining

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Expression levels of ARAF in bladder cancer cells. (A) mRNA expression of ARAF in different cell lines. (B and C) Protein expression of ARAF in different cell lines. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. ***p<0.001, ****p<0.0001 vs. SV-HUC-1.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Expressing